منابع مشابه
Establishment of LIF-Dependent Human iPS Cells Closely Related to Basic FGF-Dependent Authentic iPS Cells
Human induced pluripotent stem cells (iPSCs) can be divided into a leukemia inhibitory factor (LIF)-dependent naïve type and a basic fibroblast growth factor (bFGF)-dependent primed type. Although the former are more undifferentiated than the latter, they require signal transduction inhibitors and sustained expression of the transgenes used for iPSC production. We used a transcriptionally enhan...
متن کاملAging in iPS cells
(iPSC) technology [1] holds the promise of recreating disease in vitro in a patient-specific manner. There has been considerable success in using iPSC models to study early-onset genetic diseases, such as our own work on familial dysautonomia [2] or primary herpes simplex encephalitis [3]. The modeling of late-onset diseases in iPSC-derived lineages has been more challenging and such studies of...
متن کاملNaïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells
Quality evaluation of pluripotent stem cells using appropriate animal models needs to be improved for human regenerative medicine. Previously, we demonstrated that although the in vitro neural differentiating capacity of rabbit induced pluripotent stem cells (iPSCs) can be mitigated by improving their baseline level of pluripotency, i.e., by converting them into the so-called "naïve-like" state...
متن کاملIntegration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors
Human artificial chromosomes (HACs) have unique characteristics as gene-delivery vectors, including episomal transmission and transfer of multiple, large transgenes. Here, we demonstrate the advantages of HAC vectors for reprogramming mouse embryonic fibroblasts (MEFs) into induced pluripotent stem (iPS) cells. Two HAC vectors (iHAC1 and iHAC2) were constructed. Both carried four reprogramming ...
متن کامل[Evaluation of human iPS cells by neural differentiation and tumorigenicity].
Evaluation of human iPS cells by neural differentiation and tumorigenicity Yohei Okada, M.D., Ph.D.and Hideyuki Okano, M.D., Ph.D. Kanrinmaru Project, School of Medicine, Keio University Department of Physiology, School of Medicine, Keio University (Clin Neurol 2011;51:1079-1080)
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science-Business eXchange
سال: 2013
ISSN: 1945-3477
DOI: 10.1038/scibx.2013.1282